Am J Cancer Res 2012;2(2):xxx-xxx
Original Article
Multiple sites of highly amplified DNA sequences detected by molecular
cytogenetic analysis in HS-RMS-2, a new pleomorphic rhabdomyosarcoma cell
line
Eiji Takaoka, Hiroshi Sonobe, Kunihiro Akimaru, Shuji Sakamoto, Taro Shuin, Masanori Daibata, Takahiro Taguchi, Akira
Tominaga
Division of Human Health and Medical Science, Graduate School of Kuroshio Science, Kochi University, Nankoku, Kochi
783-8505, Japan; Department of Clinical Laboratory, Fukuyama National Hospital, Hiroshima 720-0825, Japan; Japan Science
and Technology Agency, Kami, Kochi 782-8502, Japan; Laboratory of Molecular Biology, The Science Research Center, Kochi
University, Nankoku, Kochi 783-8505, Japan; Department of Urology, Kochi Medical School, Nankoku, Kochi 783-8505, Japan;
Department of Microbiology and Infection, Kochi Medical School, Nankoku, Kochi 783-8505, Japan; Department of Medical
Laboratory, Kochi-Gakuen College, Asahi-Tenjin, Kochi 780-0955, Japan
Received January 29, 2012; accepted February 16, 2012; Epub February 19, 2012; Published February 28, 2012
Abstract: A molecular cytogenetic analysis was performed on HS-RMS-2, a cell line established in this laboratory from a rare
pleomorphic type of rhabdomyosarcoma. G-banding and multicolor-FISH analyses revealed that the cells have a complex
chromosomal composition. Comparative genomic in situ hybridization (CGH) detected eight highly amplified regions at
1p36.1-p36.2, 1p31-p32, 1q21-q31, 8q12-q21, 8q24-qter, 11q12-q13, 12q13-q14 and 18q12-q22, suggesting the co-existence
of multiple amplified oncogenes in these tumor cells. Reverse chromosome painting, using a probe regenerated by
microdissection of a long marker chromosome, revealed the native location of three of eight possible genes to be on
chromosomes 1p31-32, 12q14 and 18q21. FISH using BAC and cosmid probes revealed amplification of JUN (1p31), MYC
(8q24), CCND1 (11q13), INT2 (11q13.3), MDM2 (12q14.3-q15) and MALT (18q21). These findings indicate that at least eight
amplified oncogenes may contribute to the pathogenesis of a rare pleomorphic type of rhabdomyosarcoma. This new cell line
should prove useful for in vitro preclinical studies of molecularly targeted therapies. (AJCR00000107).
Keywords: Rhabdomyosarcoma, oncogenes, homogeneously staining region, co-amplification, FISH, CGH
Address all correspondence to:
Dr. Takahiro Taguchi
Division of Human Health and Medical Science
Graduate School of Kuroshio Science, Kochi University
Nankoku, Kochi 783-8505, Japan.
Tel: (+81) 88 880 2580; Fax: (+81) 88 880 2580
E-mail: ttaguchi@kochi-u.ac.jp
Original Article
Multiple sites of highly amplified DNA sequences detected by molecular
cytogenetic analysis in HS-RMS-2, a new pleomorphic rhabdomyosarcoma cell
line
Eiji Takaoka, Hiroshi Sonobe, Kunihiro Akimaru, Shuji Sakamoto, Taro Shuin, Masanori Daibata, Takahiro Taguchi, Akira
Tominaga
Division of Human Health and Medical Science, Graduate School of Kuroshio Science, Kochi University, Nankoku, Kochi
783-8505, Japan; Department of Clinical Laboratory, Fukuyama National Hospital, Hiroshima 720-0825, Japan; Japan Science
and Technology Agency, Kami, Kochi 782-8502, Japan; Laboratory of Molecular Biology, The Science Research Center, Kochi
University, Nankoku, Kochi 783-8505, Japan; Department of Urology, Kochi Medical School, Nankoku, Kochi 783-8505, Japan;
Department of Microbiology and Infection, Kochi Medical School, Nankoku, Kochi 783-8505, Japan; Department of Medical
Laboratory, Kochi-Gakuen College, Asahi-Tenjin, Kochi 780-0955, Japan
Received January 29, 2012; accepted February 16, 2012; Epub February 19, 2012; Published February 28, 2012
Abstract: A molecular cytogenetic analysis was performed on HS-RMS-2, a cell line established in this laboratory from a rare
pleomorphic type of rhabdomyosarcoma. G-banding and multicolor-FISH analyses revealed that the cells have a complex
chromosomal composition. Comparative genomic in situ hybridization (CGH) detected eight highly amplified regions at
1p36.1-p36.2, 1p31-p32, 1q21-q31, 8q12-q21, 8q24-qter, 11q12-q13, 12q13-q14 and 18q12-q22, suggesting the co-existence
of multiple amplified oncogenes in these tumor cells. Reverse chromosome painting, using a probe regenerated by
microdissection of a long marker chromosome, revealed the native location of three of eight possible genes to be on
chromosomes 1p31-32, 12q14 and 18q21. FISH using BAC and cosmid probes revealed amplification of JUN (1p31), MYC
(8q24), CCND1 (11q13), INT2 (11q13.3), MDM2 (12q14.3-q15) and MALT (18q21). These findings indicate that at least eight
amplified oncogenes may contribute to the pathogenesis of a rare pleomorphic type of rhabdomyosarcoma. This new cell line
should prove useful for in vitro preclinical studies of molecularly targeted therapies. (AJCR00000107).
Keywords: Rhabdomyosarcoma, oncogenes, homogeneously staining region, co-amplification, FISH, CGH
Address all correspondence to:
Dr. Takahiro Taguchi
Division of Human Health and Medical Science
Graduate School of Kuroshio Science, Kochi University
Nankoku, Kochi 783-8505, Japan.
Tel: (+81) 88 880 2580; Fax: (+81) 88 880 2580
E-mail: ttaguchi@kochi-u.ac.jp
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American Journal of Cancer Research
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